Kurzfassung

In the last decade much research has been performed on microRNAs isolated from various tissues. They were transfected, tested in different cell lines and tried to link to various diseases. However, no research was performed concerning skeletal muscle, preadipocytes or atrophic primary cells. Our proposed solution is to perform expression analyses of microRNAs in skeletal muscle, fat and atrophic tissue directly extracted from patients undergoing spinal surgery. We will define and characterize...In the last decade much research has been performed on microRNAs isolated from various tissues. They were transfected, tested in different cell lines and tried to link to various diseases. However, no research was performed concerning skeletal muscle, preadipocytes or atrophic primary cells. Our proposed solution is to perform expression analyses of microRNAs in skeletal muscle, fat and atrophic tissue directly extracted from patients undergoing spinal surgery. We will define and characterize the expression of microRNAs in various stages of muscle atrophy (healthy muscle -  mixed tissue – atrophic muscle – fat tissue). Thereby we will identify which microRNAs are up- or downregulated ad influence their target genes and thereby the relevantpathways as previously described.
After first expression analyses we will isolate primary cells from tissue extracted during surgery, infect them with cloned microRNAs and therebyenhance, block or reverse muscle atrophy. With regard to repress the influence of microRNAs and their target genes which cause atrophic conditions in vitro we will transfer identified microRNAs into our different primary cell types followed by analyzing effects with specific molecular techniques, for example Luciferase Reporter Assay/qRT-PCR.
 » weiterlesen» einklappen