Kurzfassung

Soft tissue complications are clinically relevant
problems after osteosynthesis of fractures. The goal is to
develop a method for reduction of fibroblast adhesion and
proliferation on titanium implant surfaces by plasma
polymerisation of the organo-silicon monomer hexamethyldisiloxane
(HMDSO). HMDSO was deposited under
continuous wave conditions in excess oxygen (ppHMDSO
surface) and selected samples were further modified with
an additional oxygen plasma (ppHMDSO ?  O2  surface).
Surface...Soft tissue complications are clinically relevant
problems after osteosynthesis of fractures. The goal is to
develop a method for reduction of fibroblast adhesion and
proliferation on titanium implant surfaces by plasma
polymerisation of the organo-silicon monomer hexamethyldisiloxane
(HMDSO). HMDSO was deposited under
continuous wave conditions in excess oxygen (ppHMDSO
surface) and selected samples were further modified with
an additional oxygen plasma (ppHMDSO ?  O2  surface).
Surface characterization was performed by scanning electron
microscopy, profilometry, water contact angle measurements,
infrared reflection absorption spectroscopy and
X-ray photoelectron spectroscopy. In our experimental
setup the mechanical properties, roughness and topography
of the titanium were preserved, while surface chemistry
was drastically changed. Fibroblast proliferation was
assessed by alamarBlue assay, cell morphology by confocal
microscopy visualization of eGFP-transducted fibroblasts,
and cell viability by Annexine V/propidium iodide
assay. Both modified surfaces, non-activated hydrophobic
ppHMDSO and activated hydrophilic ppHMDSO ?  O2
 were able to dramatically reduce fibroblast colonization
and proliferation compared to standard titanium. However,
this effect was more strongly pronounced on the hydrophobic
ppHMDSO surface, which caused reduced cell
adhesion and prevented proliferation of fibroblasts. The
results demonstrate that plasma modifications of titanium
using HMDSO are valuable candidates for future developments
in anti-adhesive and anti-proliferative coatings for
titanium fracture implants.
 » weiterlesen» einklappen