Kurzfassung

Obligate precondition for successful dental implant osseointegration is the development of
timely and sufficient peri-implant neovascularisation (1). It is widely accepted that especially
endothelial progenitor cells (EPCs) play an essential role to trigger blood supply and vessel
ingrowth during soft and hard tissue regeneration and wound healing processes. Adequate
stimuli induce secretion of relevant cytokines for subsequent maturation and formation of
endothelial cells (2, 3) as well as...Obligate precondition for successful dental implant osseointegration is the development of
timely and sufficient peri-implant neovascularisation (1). It is widely accepted that especially
endothelial progenitor cells (EPCs) play an essential role to trigger blood supply and vessel
ingrowth during soft and hard tissue regeneration and wound healing processes. Adequate
stimuli induce secretion of relevant cytokines for subsequent maturation and formation of
endothelial cells (2, 3) as well as differentiation of osteogenic precursors (LIT).
Specific Ti surface modifications, such as sandblasted acid-etched (SLA) surfaces, intend to
improve biocompatibility and have extensively been investigated using in vitro osteogenic
mono-cell-culture experiments. Novel alloys consisting of Ti-ZrSiO4 intend to enhance
mechanical in vivo stability. Further enhancement of surface energy of various Ti surfaces
(modSLA) showed increased protein adsorption as well as improved osteogenic cell responses.
Reviewing current literature, no systematic in vitro investigation exists on (I) EPC interactions
with high energy titanium surfaces and (II) the impact of this activation for subsequent
osteogenic cell maturation.

Objective of our proposal is to test the hypothesis that surface roughness and surface energy
promote EPC activation with subsequent osteogenic cell maturation in a synergistic manner.

Our study design provides preliminary surface characterization via SEM and EDX analysis. Cell
culture experiments include (I) quantification of cytokine (SDF-1, VEGF, IGF and HGF) release
by EPCs on the various surfaces, (II) visualization of EPC – osteoblast co-culture interactions
via lentiviral GFP and RFP transduction and CLSM detection and (III) assessment of EPC-
dependent osteogenic cell maturation in a co-culture model.

Together with existing in vitro data of our research group, the results of this study will allow a
closer understanding of the initial dental implant healing cascade. The establishment of the
intended experimental setup might serve as a tool to identify promising future surface
modifications.

Keywords:
Angiogenesis, Endothelial Progenitor Cell (EPC), cytokine release, osteogenic cell maturation,
high energy surfaces, Confocal Laser Scanning Microscopy



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