Kurzfassung

Circulating CD34+ progenitor cells (CD34+PCs) gained importance in the field of regenerative medicine due to their potential to home in on injury sites and differentiate into cells of both endothelial and osteogenic lineages. In this study, we analysed the mobilisation kinetics and the number of CD34+, CD31+, CD45+, and CD133+ cells in twenty polytrauma patients (n = 13 male, n = 7 female, mean age 46.5 17.2 years, mean injury severity score (ISS) 35.8 12.5 points). In addition, the...Circulating CD34+ progenitor cells (CD34+PCs) gained importance in the field of regenerative medicine due to their potential to home in on injury sites and differentiate into cells of both endothelial and osteogenic lineages. In this study, we analysed the mobilisation kinetics and the number of CD34+, CD31+, CD45+, and CD133+ cells in twenty polytrauma patients (n = 13 male, n = 7 female, mean age 46.5 ± 17.2 years, mean injury severity score (ISS) 35.8 ± 12.5 points). In addition, the endothelial differentiation capacity of enriched CD34+PCs was assessed by analysing Dil-ac-LDL/lectin uptake, the expression of endothelial markers, and the morphological characteristics of these cells in Matrigel® and spheroid cultures. We found that on days 1, 3, and 7 after a major trauma, the number of CD34+PCs increased 2.2-fold (p<0.0001) over the number of CD34+PCs from a control population of healthy, age-matched volunteers. The number of CD31+ cells were consistently higher on days 1 (1.4-fold, p<0.01) and 7 (1.3-fold, p<0.01), whereas the changes in the CD133+ cell levels were not considered to be significant. The endothelial differentiation of CD34+PCs was significantly induced in the polytrauma patients. In addition, we show that the CD34+PC levels in severely injured patients were not correlated with clinical parameters, such as the ISS score, the acute physiology and chronic health evaluation II score (APACHE
II), as well as the sequential organ failure assessment score (SOFA-2). Our results clearly indicate that pro-angiogenic cells are systemically mobilized after polytrauma and that their numbers are sufficient for the development of novel therapeutic models in regenerative medicine.
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