Kurzfassung

Ketamine can be used as a pharmacological tool to decode its resilience-promoting properties. In previous studies on the time-dependent hippocampal proteome and metabolite profile of ketamine-treated mice we examined total hippocampal homogenates, thus not allowing to specifically select and isolate cells activated by ketamine. To selectively target neuronal subpopulations activated by ketamine, we have established the TRAP (Targeted Recombination in Active Populations) approach. This allows...Ketamine can be used as a pharmacological tool to decode its resilience-promoting properties. In previous studies on the time-dependent hippocampal proteome and metabolite profile of ketamine-treated mice we examined total hippocampal homogenates, thus not allowing to specifically select and isolate cells activated by ketamine. To selectively target neuronal subpopulations activated by ketamine, we have established the TRAP (Targeted Recombination in Active Populations) approach. This allows to selectively isolate the subset of neurons targeted by ketamine and to measure transcriptome and epigenetic features of resilience-promoting mechanisms including chromatin accessibility (ATAC) in combination with next-generation sequencing.» weiterlesen» einklappen