Inhaltszusammenfassung

Biogeochemical interfaces in soil are dynamic in the spatial and temporal domain and require advanced visualization and quantification tools to link in-vitro experiments with natural systems. This study presents the development, characterization, and application of functional nanoparticles coated with monoclonal antibodies to visualize the distribution of benzo[a]pyrene in porous media using magnetic resonance imaging. The labeled particles are 450 nm in diameter and interact...Biogeochemical interfaces in soil are dynamic in the spatial and temporal domain and require advanced visualization and quantification tools to link in-vitro experiments with natural systems. This study presents the development, characterization, and application of functional nanoparticles coated with monoclonal antibodies to visualize the distribution of benzo[a]pyrene in porous media using magnetic resonance imaging. The labeled particles are 450 nm in diameter and interact with benzo[a]pyrene covalently bound to silanized silica gel. They did not bind to benzo[a]pyrene adsorbed to plain silica gel. Although unspecific filtration was low, washing steps are required for visualization. The ability to visualize benzo[a]pyrene is inversely correlated to the heterogeneity of the soil materials. There are access restrictions to narrow pore spaces which allow to visualize only those pathways which are also accessible to bacteria and hydrocolloids. The production of the particles is applicable to other antibodies which extends the range of potential target contaminants.» weiterlesen» einklappen

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