Starten Sie Ihre Suche...


Durch die Nutzung unserer Webseite erklären Sie sich damit einverstanden, dass wir Cookies verwenden. Weitere Informationen

Components of the plasminogen activation system promote engraftment of porous polyethylene biomaterial via common and distinct effects

PLoS one. Bd. 10. H. 2. Lawrence, Kan.: PLoS 2014 e0116883

Erscheinungsjahr: 2014

ISBN/ISSN: 1932-6203

Publikationstyp: Zeitschriftenaufsatz

Sprache: Englisch

Doi/URN: 10.1371/journal.pone.0116883

Volltext über DOI/URN

GeprüftBibliothek

Inhaltszusammenfassung


Rapid fibrovascularization is a prerequisite for successful biomaterial engraftment. In addition to their well-known roles in fibrinolysis, urokinase-type plasminogen activator (uPA) and tissue plasminogen activator (tPA) or their inhibitor plasminogen activator inhibitor-1 (PAI-1) have recently been implicated as individual mediators in non-fibrinolytic processes, including cell adhesion, migration, and proliferation. Since these events are critical for fibrovascularization of biomaterial, w...Rapid fibrovascularization is a prerequisite for successful biomaterial engraftment. In addition to their well-known roles in fibrinolysis, urokinase-type plasminogen activator (uPA) and tissue plasminogen activator (tPA) or their inhibitor plasminogen activator inhibitor-1 (PAI-1) have recently been implicated as individual mediators in non-fibrinolytic processes, including cell adhesion, migration, and proliferation. Since these events are critical for fibrovascularization of biomaterial, we hypothesized that the components of the plasminogen activation system contribute to biomaterial engraftment. Employing in vivo and ex vivo microscopy techniques, vessel and collagen network formation within porous polyethylene (PPE) implants engrafted into dorsal skinfold chambers were found to be significantly impaired in uPA-, tPA-, or PAI-1-deficient mice. Consequently, the force required for mechanical disintegration of the implants out of the host tissue was significantly lower in the mutant mice than in wild-type controls. Conversely, surface coating with recombinant uPA, tPA, non-catalytic uPA, or PAI-1, but not with non-catalytic tPA, accelerated implant vascularization in wild-type mice. Thus, uPA, tPA, and PAI-1 contribute to the fibrovascularization of PPE implants through common and distinct effects. As clinical perspective, surface coating with recombinant uPA, tPA, or PAI-1 might provide a novel strategy for accelerating the vascularization of this biomaterial.» weiterlesen» einklappen

Autoren


Reichel, Christoph A. (Autor)
Hessenauer, Maximilian E. T. (Autor)
Pflieger, Kerstin (Autor)
Rehberg, Markus (Autor)
Kanse, Sandip M. (Autor)
Zahler, Stefan (Autor)
Krombach, Fritz (Autor)
Berghaus, Alexander (Autor)
Strieth, Sebastian (Autor)

Klassifikation


DDC Sachgruppe:
Medizin