Inhaltszusammenfassung

We tested the performance of buccal swabs for microsatellite analysis in an introduced population of the Common Wall Lizard (P. muralis) in Germany. The quantity and quality of the isolated DNA collected by buccal swabbing and by screwing a tail tip of the same individuals was compared. Although the DNA yield from buccal swabs was much lower than from tissue, it was sufficient for a successful amplification. We genotyped the individuals at two microsatellite loci. Buccal swabs generated genot...We tested the performance of buccal swabs for microsatellite analysis in an introduced population of the Common Wall Lizard (P. muralis) in Germany. The quantity and quality of the isolated DNA collected by buccal swabbing and by screwing a tail tip of the same individuals was compared. Although the DNA yield from buccal swabs was much lower than from tissue, it was sufficient for a successful amplification. We genotyped the individuals at two microsatellite loci. Buccal swabs generated genotypes just as well as tissue samples. We could not find a lower threshold of DNA quantity that increased genotyping errors. In contrast, very high DNA yields (>10 ng/ml), as found in some tissue samples, produced a higher number of unspecific peaks. These results show that buccal swabs are a simple and efficient non-invasive sampling method for DNA analysis in adult lacertid lizards. Carefully applied, the technique does not harm the specimens in their locomotor performance and energy reserves. An additional advantage of buccal swabbing is the time-saving DNA extraction, since there is no need to remove scales, chop up the tissue, nor for a long digestion step. » weiterlesen» einklappen

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