Kurzfassung

Recent clinical and animal studies have shown that extracorporal shock wave therapy (ESWT) has a positive influence on the healing process of musculoskeletal disorders. The underlying biological effects of ESWT on human skeletal muscle cells have not yet been investigated. In this study we investigated human skeletal muscle cells after exposure to radial extracorporal shock waves (rESWs). Cells were isolated from muscle specimens taken from adult patients undergoing spine surgery. Muscle...Recent clinical and animal studies have shown that extracorporal shock wave therapy (ESWT) has a positive influence on the healing process of musculoskeletal disorders. The underlying biological effects of ESWT on human skeletal muscle cells have not yet been investigated. In this study we investigated human skeletal muscle cells after exposure to radial extracorporal shock waves (rESWs). Cells were isolated from muscle specimens taken from adult patients undergoing spine surgery. Muscle cells were exposed to rESWs in vitro with different energy flux densities (EFD). Cell viability and gene expression of the paired box protein 7 (Pax7), neural cell adhesion molecule (NCAM) and myogenic factor 5 (Myf5) as muscle cell markers were compared to non-treated muscle cells that served as controls. Isolated muscle cells were positive for the hallmark protein of satellite cells, Pax7, NCAM and Myf5. Exposure to rESWs at low EFD enhanced cell viability whereas higher EFD had no significant impact on cell viability. Gene expression of Pax7 was up-regulated after exposure to higher EFD, whereas Pax7, NCAM and Myf5 gene expression was down-regulated after exposure to even higher EFD. This is the first study demonstrating that rESWT has the potential to modulate the biological function of human skeletal muscle cells.
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