Kurzfassung

Although in the last years plenty of research has been performed concerning bone tissue engineering, the perfect scaffold especially for fractures of critical sizes still has not been defined. Polysaccharide-based hydrogels demonstrate optimal properties and fulfill the main requirements for a scaffold as they are hydrophilic, biocompatible, and biodegradable. Amylose, pullulan, dextran – polysaccharides in varying mixtures were synthesized and photocrosslinked. After swelling, they were...Although in the last years plenty of research has been performed concerning bone tissue engineering, the perfect scaffold especially for fractures of critical sizes still has not been defined. Polysaccharide-based hydrogels demonstrate optimal properties and fulfill the main requirements for a scaffold as they are hydrophilic, biocompatible, and biodegradable. Amylose, pullulan, dextran – polysaccharides in varying mixtures were synthesized and photocrosslinked. After swelling, they were mechanically stable, and contain pores, whose size can be modulated. Moreover it was possible to bind growth factors, SDF1 and BMP2 cytokines covalently to polysaccharide hydrogels to enforce their biological desired properties.
As skeletal development depends on angiogenesis, we employed mono and co-culture of human primary osteoblasts (hOB) models with human endothelial cells (Huvec) in order to analyze cell–hydrogel communications and their influence on each other. Our aim is to develop a prevascularized scaffold, which could interact with host tissue and supply it with nutrients and oxygen. Both employed cell types grew on all tested hydrogels, which was tested microscopically and using prolifearation assays. Migration assays, real-time PCR demonstrated an enhanced expression of cell specific markers after binding of growth factors and demonstrated their functional bioactivity after binding and release from hydrogels. Our results show the potential for hydrogels as a biomaterial in various applications.
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