Kurzfassung

Orthopedic implant failure due to aseptic loosening and mechanical instability is still a major problem in total joint replacement. Improving osseointegration at the bone-implant-interface may reduce relative motion and thus particle-induced loosening. Bone sialoprotein (BSP) possesses osteoinductive properties both when coated onto titanium femoral implants and in rat calvarial models. In this study, we investigate and develop an optimal method of coating titanium implants with BSP using...Orthopedic implant failure due to aseptic loosening and mechanical instability is still a major problem in total joint replacement. Improving osseointegration at the bone-implant-interface may reduce relative motion and thus particle-induced loosening. Bone sialoprotein (BSP) possesses osteoinductive properties both when coated onto titanium femoral implants and in rat calvarial models. In this study, we investigate and develop an optimal method of coating titanium implants with BSP using different chemical processing methods (physisorbtion, PIRANHA-surface activation, aminosilane linker (APTES), etc.). The modified surfaces are being investigated in cell culture of primary human osteoblasts (HOb) and L929 mouse fibroblasts. Gene expression of specific bone turnover markers is detected at different time intervals. We found that BSP had an suppressive effect on HOb during the early phases of migration, adhesion and proliferation irrespective of its concentration or coating method. Although alkaline phosphatase activity was reduced after 4 days, Alizarin Red S staining showed an increased calcium deposition after 21 days. Especially osteopontin (OPN) and Runt-related transcription factor 2 (RUNX2) were upregulated by BSP. The improvement of calcium deposition and stimulation of cell differentiation demonstrate the capability of BSP as a surface modificator that might enhance osseointegration in orthopedic implants.
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