Kurzfassung

The fast development of advanced RP/SSF techniques has started to revolutionize surgery, orthopaedics and dentistry. These techniques allow the fabrication of customized scaffolds/implants that meet the demands of individual patients. It would be of great advantage to have a scaffold material that does not require supplementation with exogenous growth factors / cytokines in order to achieve a sufficient response of cells involved in hydroxyapatite (HA) formation. In bone tissue engineering,...The fast development of advanced RP/SSF techniques has started to revolutionize surgery, orthopaedics and dentistry. These techniques allow the fabrication of customized scaffolds/implants that meet the demands of individual patients. It would be of great advantage to have a scaffold material that does not require supplementation with exogenous growth factors / cytokines in order to achieve a sufficient response of cells involved in hydroxyapatite (HA) formation. In bone tissue engineering, these factors are needed by human mesenchymal stems cells (hMSCs), taken e.g. from the iliac crest and seeded onto an inert scaffold, for differentiation into osteoblasts. It would be a great success if it would be possible to get the differentiating cells to produce these factors by themselves. The morphogenetically active inorganic bio-polymers, biosilica and bio-polyP, offer a new strategy: these polymers are able to elicit the expression and release of morphogen/ligand molecules, such as BMP-2 and RANKL, and thereby promote cell differentiation and HA deposition. As a suitable, morphogenetically active and biocompatible matrix for 3D cell printing, we succeeded to develop a biosilica-containing alginate hydrogel.
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