Kurzfassung

Obligate precondition for successful dental implant osseointegration is the development of timely and sufficient peri-implant neovascularisation (1). It is widely accepted that especially endothelial progenitor cells (EPCs) play an essential role to trigger blood supply and vessel ingrowth during soft and hard tissue regeneration and wound healing processes. Adequate stimuli induce secretion of relevant cytokines for subsequent maturation and formation of endothelial cells (2, 3) as well as...Obligate precondition for successful dental implant osseointegration is the development of timely and sufficient peri-implant neovascularisation (1). It is widely accepted that especially endothelial progenitor cells (EPCs) play an essential role to trigger blood supply and vessel ingrowth during soft and hard tissue regeneration and wound healing processes. Adequate stimuli induce secretion of relevant cytokines for subsequent maturation and formation of endothelial cells (2, 3) as well as differentiation of osteogenic precursors (LIT).
Specific Ti surface modifications, such as sandblasted acid-etched (SLA) surfaces, intend to improve biocompatibility and have extensively been investigated using in vitro osteogenic mono-cell-culture experiments. Novel alloys consisting of Ti-ZrSiO4 intend to enhance mechanical in vivo stability. Further enhancement of surface free energy of hydrophilic SLA Ti surfaces (modSLA) showed increased protein adsorption as well as improved osteogenic cell responses. Reviewing current literature, no systematic in vitro investigation exists on (I) EPC interactions with high energy titanium surfaces and (II) the impact of this activation for subsequent osteogenic cell maturation. Objective of our proposal is to test the hypothesis that surface roughness and surface energy promote EPC activation with subsequent osteogenic cell maturation in a synergistic manner. Our study design provides preliminary surface characterization via SEM and EDX analysis[-1] . Cell culture experiments include (I) quantification of cytokine (SDF-1, VEGF, IGF and HGF) release by EPCs on the various surfaces, (II) visualization of EPC – osteoblast co-culture interactions via lentiviral GFP and RFP transduction and CLSM detection and (III) assessment of EPC-dependent osteogenic cell maturation in a co-culture model. Together with existing in vitro data of our research group, the results of this study will allow a closer understanding of the initial dental implant healing cascade. The establishment of the intended experimental setup might serve as a tool to identify promising future surface modifications.» weiterlesen» einklappen