Kurzfassung

Intraperitoneal adhesions represent a major problem after abdominal surgery with serious clinical complications. In the present study we analysed the histomorphological and ultrastructural changes after implantation of four different barriers with view of their anti-adhesive effects and biocompatibility in a standardized rat model.
Standardised peritoneal lesions were created in Wistar rats using electrocautery and suturing. The lesions were treated with Seprafilm (n=30), Intercoat (n=30),...Intraperitoneal adhesions represent a major problem after abdominal surgery with serious clinical complications. In the present study we analysed the histomorphological and ultrastructural changes after implantation of four different barriers with view of their anti-adhesive effects and biocompatibility in a standardized rat model.
Standardised peritoneal lesions were created in Wistar rats using electrocautery and suturing. The lesions were treated with Seprafilm® (n=30), Intercoat® (n=30), Adept® (n=30), Spraygel® (n=30) and without barrier (n=30). Adhesion formation was evaluated 14 days after surgery. The materials with peritoneal tissue were studied histologically and by scanning electron microscopy.
Histologically animals treated with Seprafilm showed areas with activated mesothelial cells, slight oedema and granulocytic infiltration in the subserosa. In animals treated with Intercoat the serosa showed fibrous tissue with minimal lymphocytic infiltrates and resting mesothelial cells. Animals treated with Adept showed areas with activated mesothelial cells and an infiltration by inflammatory cells. Animals treated with Spraygel® had a serosa lined by flat mesothelial cells. In the subseros there was marked oedema with severe inflammation as evidenced by abscess formation.
Differences in the material-tissue Interaction are evident between the used barriers. For the anti-adhesive effect and the biocompatibility the mesothelial cells seemed to be important, which should be clarified in further investigations.» weiterlesen» einklappen