Kurzfassung

Classical work has demonstrated that hippocampal neurons synthesize new proteins from locally stored RNA at synapses in response to stimuli within minutes. The aim of the present study is to use mouse brain slices and cell cultures to disclose the local total protein synthesis as well as defined protein synthesis that we have identified to be locally translated in response to glutamate stimulation (in vitro) or neuronal activity (in vivo) in oligodendrocyte precursor cells (OPCs)/NG2 glia...Classical work has demonstrated that hippocampal neurons synthesize new proteins from locally stored RNA at synapses in response to stimuli within minutes. The aim of the present study is to use mouse brain slices and cell cultures to disclose the local total protein synthesis as well as defined protein synthesis that we have identified to be locally translated in response to glutamate stimulation (in vitro) or neuronal activity (in vivo) in oligodendrocyte precursor cells (OPCs)/NG2 glia within the CNS. The two particularly interesting RNAs are a transcription factor and a specific variant histone. The local translation of this variant histone protein in response to glutamate suggests a role in epigenetic modulation of NG2 glia function by neuronal activity. Methods include immunoisolated NG2 glia cells in vitro and the use of FISH, puromycylation/PLA to detect new synthesis of defined proteins and BONCAT, a technique to visualize global protein synthesis, and electrophysiological in vitro recordings.
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